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Makanya sewaktu meninggal dunia, mereka biasanya dipakaikan selimut berwarna merah dan lilin yang berwarna merah, sebagai perlambang “bahagia”.
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m noticed for the different metals Keep to the trend: Zn = Co > Mn > Cd > Fe > Ni . The Fe that contains enzyme wasn't thoroughly reconstituted with steel and thus the kinetic constants may very well be larger sized Should the metal internet site was completely occupied. The values for k
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Reconstitution of the apo-enzymes was done link alternatif naga26 right away at 4 °C right before elimination of unbound metal by passage via a PD-10 column. The PD-ten column was pre-addressed with dipicolinate to eliminate traces of unbound metal and after that washed with five column volumes of metallic cost-free HEPES buffer, pH eight.0. Just after elution, the focus of the metallic-reconstituted enzyme was resolute by UV absorbance and also the metal content material from the samples determined by ICP-MS.
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